_1728186322 20241006034522000 Rovedar Daryoushbabazadeh@gmail.com Rovedar Farm Animal Health and Nutrition Farm Anim. Health Nutr. 2980-7905 09 03 2024 3 3 Aluor Adum https://orcid.org/0009-0005-3477-7875 Lawrence Ademu https://orcid.org/0000-0003-1806-8508 Introduction: Heat stress has become a significant concern for the global poultry industry as a result of climate change. This condition can adversely affect growth, gut health, immune function, production levels, and reproductive performance, and can lead to lipid peroxidation in poultry. Therefore, this study aimed to evaluate Interleukin 6 and 10 immune responses, anti-oxidant indices, serum biochemistry, and hepatic and splenic histopathology of heat-stressed broiler chickens supplemented with α-lipoic acid (α-LA) in their diet. Materials and methods: A total of two hundred and four unsexed day-old Arbor acre broiler chicks were randomly divided into four treatment groups and replicated three times to receive α-LA at 0, 50, 100, and 200 mg/kg, respectively in a completely randomized design for 42 days after a one-week adjustment period. The average temperature in the morning and afternoon was 27.9℃ and 35.1℃, respectively, while the morning and afternoon humidity was 73.1% and 44.1% respectively. Feed and water were provided ad libitum. At the end of the feeding trial, samples were taken for cytokines (interleukin-6 and 10), anti-oxidant, serum biochemistry, and hepatic and splenic histopathology analyses.  Results: An inverse relationship was observed between pro-inflammatory (IL-6) and anti-inflammatory (IL-10) cytokines whereby, IL-6 levels decreased while IL-10 levels increased relative to increasing α-LA levels in treatment groups. The treatment groups also indicated an increasing trend with rising levels of α-LA for superoxide dismutase, catalase, glutathione, and total antioxidant capacity. The α-LA significantly influenced malondialdehyde production, showing its reduction with rising levels of α-LA. A decrease in serum glucose and low-density lipoproteins was observed with increasing levels of α-LA, while high-density lipoproteins increased with increasing levels of α-LA. Cholesterol, triglycerides, very low-density lipoproteins, aspartate transaminase, alanine transaminase, and alkaline phosphate remained unchanged across the treatment groups. Conclusion: α-LA supplementation at 200mg/kg in diet had the highest effects on immune responses of interleukin 6 and 10, antioxidant, serum biochemical indices, and histopathology of heat-stressed broiler chickens.        09 03 2024 57 63 https://creativecommons.org/licenses/by/4.0 10.58803/fahn.v3i3.57 https://fahn.rovedar.com/index.php/FAHN/article/view/57 https://fahn.rovedar.com/index.php/FAHN/article/download/57/82 https://fahn.rovedar.com/index.php/FAHN/article/download/57/82 Asres A, and Amha N. Effect of stress on animal health: A review. J Biol Agric Healthc. 2014; 4(27): 116-122. Available at: https://core.ac.uk/download/pdf/234660471.pdf 10.2141/jpsa.0150062 10.1016/j.psj.2023.102814 10.1016/j.ajme.2017.09.001 El Barky AR, Hussein SA, and Mohamed TM. The potent antioxidant alpha lipoic acid. J Plant Chem Ecophysiol. 2017; 2(1): 1016. Available at: https://austinpublishinggroup.com/jpceonline/fulltext/jpce-v2-id1016.php 10.1016/S0891-5849(97)00371-7 10.1111/jpn.12693 National research council (NRC). Nutrient requirements of poultry. 9th Revised ed. Washington, DC, USA: National Academic Press; 1994. Available at: https://www.agropustaka.id/wp-content/uploads/ 2020/04/agropustaka.id_buku_Nutrient-Requirements-of-Poultry_Ninth-Revised-Edition-1994-NRC.pdf 10.13031/2013.12971 10.1175/JAMC-D-11-0143.1 10.1023/A:1012772311177 10.3390/ijms15045649 Lala V, Zubair M, and Minter DA. Liver function tests. Treasure Island (FL): StatPearls Publishing; 2023. PMID: 29494096 Gurina TS, and Simms L. Histology, staining. Treasure Island (FL): StatPearls Publishing; 2023. PMID: 32491595 JMP® software, version 6.12 of the SAS System for Windows. SAS Institute, Inc., Cary, NC, USA. 2012. 10.1089/jir.2014.0221 10.1101/cshperspect.a016295 10.1007/s43450-023-00370-1 10.1371/journal.pone.0153456 Murali P, and Sherin GK. Supplementation of alpha lipoic acid on serum biochemical, minerals and antioxidant status in broiler chicken fed diet with animal fat. J Entomol Zool Stud. 2020; 8(4): 1622-1626. Available at: https://www.entomoljournal.com/archives/2020/vol8issue4/PartZ/8-3-327-933.pdf 10.1016/B978-0-12-819096-8.00022-7 10.21608/ejnf.2020.148160 10.5713/ajas.2009.90101 10.1007/s12088-021-00948-0 10.3758/BF03330555 10.1093/ps/86.3.545 Pappan N, Awosika AO, Rehman A. Dyslipidemia. In: StatPearls (Internet). Treasure Island (FL): StatPearls Publishing, 2024; www.ncbi.nlm.nih.gov/books/NBK560891/ Gelatt C. Understanding cholesterol: HDL, LDL, and triglyceride. Healthlines. Riverview health blog. 2017. Available at: https://riverview.org/blog/nutrition-2/understanding-cholesterol-hdl-ldl-and-triglycerides/ 10.3382/ps/pez056 10.1016/j.psj.2022.102085 10.21608/zvjz.2022.156750.1188 10.1016/j.jtherbio.2018.04.009