Effect of a Single Injection of GnRH Analog Alone and at the Time of AI on Reproductive Performance of Nili Ravi Buffaloes

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Muhammad Binyameen
Muhammad Imran
Muhammad Waseem
Saba Anwar
Hina Tahir
Burhan Azam
Muhammad Asim Tauseef
Muhammad Saleem


Introduction: The gonadotropin-releasing hormone (GnRH) is a decapeptide hormone released from basal neurons of the hypothalamus. It stimulates the production of follicle-stimulating hormone (FSH) and luteinizing hormone (LH)  in the pituitary. The objective of the study was to evaluate the effect of a single injection of gonadotropin-releasing hormone (GnRH) analog at the time of artificial insemination (AI) on buffalo estrus, conception, pregnancy, and calving rate.

Materials and methods: The anestrus nulliparous Nili Ravi buffalo heifers (n=80) were divided into 4 groups randomly. Group A (n=20) received only a single injection of GnRH analog at start of the experiment (Day 0), group B (n=20) received a single injection of GnRH analog at Day 0 and prostaglandine (PGF2α) at Day 7, group C (n=20) received progesterone sponge for 7 days, and group D (n=20) received progesterone sponge for 7 days and PGF2α at Day 9. In 2nd study, buffaloes (n=60) expressing first (n=20), second (n=20), and third (n=20) postpartum estrus were enrolled. Moreover, heifers (n=20), expressing estrus first time were also included. Animals (n=10) from each group, received a single injection of GnRH analog at the time of AI were named as treatment group and the control (n=10) did not receive any treatment. Pregnancy tests were performed with the help of ultrasound on days 35 and 65 post-AI.

Results: The pregnancy and calving rates were lower in GnRH group compared to other treatments. Similarly, the administration of GnRH analog did not increase pregnancy rates in treatment groups.

Conclusion: The single injection of GnRH analog alone at the time of AI did not improve the reproductive performance of buffaloes.       

Article Details

How to Cite
Binyameen, M., Imran, M., Waseem, M., Anwar, S., Tahir, H., Azam, B., Tauseef, M. A., & Saleem, M. (2024). Effect of a Single Injection of GnRH Analog Alone and at the Time of AI on Reproductive Performance of Nili Ravi Buffaloes. Farm Animal Health and Nutrition, 3(1), 17–21. https://doi.org/10.58803/fahn.v3i1.38
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